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Etoposide does not affect biotinylation of K12 in histone H4 from G1 phase-arrested JAr cells. Cells were arrested in G1 phase using hydroxyurea before treatment with etoposide. Panel A: G1-arrested cells; panel B: asynchronous cells (control). Cell cycle arrest was confirmed using flow cytometry (top row), and biotinylation of K12 in histone H4 was visualized by Western analysis at timed intervals after etoposide treatment (bottom row); n.d. = not detectable.
