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. Author manuscript; available in PMC: 2005 Oct 21.
Published in final edited form as: Endocrinology. 2005 May 26;146(9):3907–3916. doi: 10.1210/en.2005-0403

Figure 3 . Human FSH- and hCG-induced aromatase mRNA in immature granulosa cells infected with different amounts of Ad-hLHR.

Figure 3

Primary cultures of immature granulosa cells were infected with adenoviral constructs as described in the legend to Figure 1. Two days after infection cells were incubated with buffer only (left panel), hFSH (middle panel) or hCG (right panel). Total RNA was then collected and used to quantify aromatase mRNA using reverse transcription followed by real-time PCR, as described in Materials and Methods.

For ease of comparison with other figures, and in order to avoid the inherent variability in absolute values associated with the use of primary cultures, all data are expressed relative to the hFSH-induced aromatase response of cells infected with βgal (see white bar in the middle panel). The levels of aromatase mRNA in cells incubated with buffer only were often undetectable (see left panel). Each bar is the mean ± SEM of 4–11 independent experiments. Means within a panel with different letters (a, b, c) are significantly different from each other (P<0.05).