Primary cultures of immature granulosa cells were infected with adenoviral constructs coding for Ad-βgal at 200 MOI (labeled βgal in the figure), a mixture of hFSHR Ad-βgal at 20 MOI plus Ad-βgal at 180 MOI (labeled hFSHR-MOI 20) or Ad-hFSHR at 200 MOI (labeled hFSHR-MOI 200). The binding of 125I-hFSH (left panel) or 125I-hCG (right panel) were measured 2 days after infection as described in Materials and Methods.
Each bar is the mean ± SEM of 5–9 independent experiments. Means within a panel with different letters (a, b, c) are significantly different from each other (P<0.05).