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. 2005 Oct 4;5:56. doi: 10.1186/1471-2180-5-56

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Copyright © 2005 Takahashi and Watanabe; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blotting with anti-meningococcal GGT rabbit antiserum [25] (A) and Coomassie Brilliant Blue staining (B) of the whole cell extracts after SDS-PAGE. Bacterial whole cell extracts equivalent to 0.025 OD₆₀₀were analyzed. Lane 1, N. meningitidis strain H44/76; lane 2, HT1089 (H44/76 Δggt::spc); lane 3, ATCC49226 (N. gonorrhoeae); lane 4, NIID54 (N. gonorrhoeae); lane 5, NIID103 (N. gonorrhoeae); lane 6, HT1195 (NIID103 Δggh::spc); lane 7, NIID106 (N. gonorrhoeae); lane 8, HT1196 (NIID106 Δggh::spc). Black arrows show the bands corresponding to the processed small and large subunits of meningococcal GGT and the gray arrow indicates the 15-kDa band corresponding to the truncated protein product of the gonococcal ggh gene. M stands for molecular weight marker.