Table 2.
Purification of AtGA3OX4 from E. coli cells (approx. 7 g)
Purification step | Protein (mg) | Specific activity (pkat/mg) | Total activity (pkat) | Apparent purification Factor | Yield (%) |
Soluble cell fraction | 790 | 360 | 284,400 | - | - |
IMAC chromatography | 4.0 | 4,325 | 17,300 | 12X | 6% |
Superdex 75 | 0.075 | 5,466 | 410 | 1.26X | 0.14% |