Fig. 3. The dynamics of developing amplicon replication compartments. Selected images of a time-lapse series of Vero cells co-infected with amplicon EYFPnlsTetR and wild-type HSV-1 helper virus are shown. The recruitment of the EYFPnlsTetR fusion protein into the amplicon replication compartment illustrates that replication is very rapid once it has been initiated. A second replication compartment starts developing at later stages of the infection. This particular cell exhibited rapid rotation during the early stages of the infection.