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. 2002 Sep 16;21(18):4841–4850. doi: 10.1093/emboj/cdf479

graphic file with name cdf479f11.jpg

Fig. 11. Cytoplasmic HET-C heterocomplexes do not always co- localize with dead hyphal compartments. (A) A DIC image of hyphae from a CJ44 (Δsp het-cPA::HA + Δsp het-cOR::GFP) incompatible transformant. (B) Transformant in (A) after staining with Evans Blue (rhodamine filter set). (C) The same field of hyphae showing labeling by anti-HA antibodies (HET-CPA::HA; Alexa 488). The white arrow in (A), (B) and (C) shows a dead hyphal compartment. The yellow arrow in (A), (B) and (C) shows a live hyphal compartment. Scale bars = 10 µm.