Fig. 2. Schematic diagram of epitope-tagged het-c constructs. N-terminal white boxes signify signal peptide deletion constructs. Patterned boxes indicate the het-c allelic specificity domain. GFP-tagged versions of het-c^OR and het-c^GR included the first 730 amino acids of HET-C^OR and 727 amino acids of HET-C^GR, which are fully functional forms (Saupe et al., 1996). The het-c^PA and het-c^OR HA-tagged constructs included DNA sequences for the nine amino acid HA-epitope tag fused to the 3′ end of the het-c open reading frame (ORF). Constructs were introduced into standard het-c tester strains C2-2-9 (het-c^OR) and C9-2 (het-c^PA) (Saupe and Glass, 1997) to test for function. C, compatible transformants; I, incompatible transformants. Differences in phenotype or het-c allelic specificity were not observed when tagged, untagged or het-c signal peptide deletion constructs were introduced into C2-2-9 or C9-2, when using either the gpd promoter or het-c promoter (see Materials and methods).