Fig. 7. Dab2 PTB domain overexpression selectively interferes with LDL uptake. COS-7 cells transiently transfected with myc-tagged Dab2 PTBx2 were pulsed for 15 min with 20 µg/ml diI-LDL alone (A–D), both diI-LDL and 25 µg/ml biotinylated transferrin (E) or transferrin alone (F–H) for 15 min prior to fixation. Transfected cells were identified using mAb 9E10 (B–D, G and H, in green in C, D and H) and transferrin with Alexa 488– (E) or Alexa 594–streptavidin (F and H). A single confocal section of triple-labeled cells is shown in (E) (myc epitope, blue; transferrin, green; LDL, red).