Fig. 5. Localization of the Mor2 mutant protein. (A–C) The nmt41–GFP–Mor2 [YS84-1 (B)] and nmt41–GFP–Mor2-282 [YY5-5 (C)] strains grown in EMM containing 8 µM thiamine were transferred into EMM medium. After cultivation for 14 h at 25°C, the cells were transferred to YPD medium at 36°C, fixed at the indicated times, and stained with rhodamine–phalloidin. The cells of the 36°C culture were taken at the indicated times for examination of the GFP–Mor2 and GFP–Mor2-282 proteins by immunoblotting (A).