Fig. 3. VE-cadherin does not require its cytoplasmic tail for association with full-length VE-PTP. (A) Schematic representation of the domain structure of full-length VE-cadherin and truncated VE-cadherin lacking its complete cytoplasmic domain (VE-cad EC). (B) COS cells were transfected either with full-length VE-PTP and full-length VE-cadherin (VE-PTP/VE-cadherin), with VE-cadherin alone (VE-cadherin), with full-length VE-PTP and truncated VE-cadherin lacking its complete cytoplasmic domain (VE-PTP/VE-cad EC) or with the truncated version of VE-cadherin alone (VE-cad EC). Cell lysates were subjected to immunoprecipitations with anti-VE-PTP antibodies, and precipitated proteins were immunoblotted either with anti-VE-cadherin antibodies (upper) or with anti-VE-PTP antibodies (middle). Note that full-length and truncated VE-cadherin were co-precipitated with VE-PTP. To control transfection efficiency, total cell lysates were immunoblotted for VE-cadherin. The asterisk marks the degradation product of VE-cadherin. Molecular mass markers (in kDa) are shown on the left.