Fig. 7. In vivo dephosphorylation of VE-cadherin by Flag-VE-PTP. COS cells were transfected with VE-cadherin, VEGFR-2 (Flk-1) and Flag-VE-PTP (lane 1); with VE-cadherin, VEGFR-2 (Flk-1) and the dominant negative Flag-VE-PTP R/A mutant (lane 2); with VE-cadherin and VEGFR-2 (Flk-1) (lane 3); or with VE-cadherin alone (lane 4). Cell lysates were immunoprecipitated with either anti-VE-cadherin, anti-VEGFR-2 (Flk-1) or anti-Flag antibodies, and immunoprecipitates were analysed by immunoblotting. Note that VE-cadherin was only phosphorylated in the presence of Flk-1 and that this phosphorylation was reversed by recombinant VE-PTP, but not by its point-mutated version. Molecular mass markers (in kDa) are shown on the left.