Table 1.

Primary and secondary antibodies used for immunofluorescence analysis.

Antibodies	Dilution	Incubation condition
Mouse anti-α-Actin (Sigma-Aldrich, Saint Louis, USA)	1:100	O.N. at 4 °C
Rabbit anti-alpha smooth muscle (Abcam, Cambridge, UK)	1:100	O.N at 4 °C
Rabbit anti-Vimentin (Abcam, Cambridge, UK)	1:100	O.N at 4 °C
Mouse anti-Occludin Monoclonal Antibody (Invitrogen, USA)	1:100	O.N at 4 °C
Mouse anti-E-cadherin (Santa Cruz Biotechnology, Dallas, USA)	1:100	O.N at 4 °C
Rabbit anti-collagen I (Abcam, Cambridge, UK)	1:100	O.N at 4 °C
Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor 488 (Invitrogen, Carlsbad, USA)	1:200	1 h at RT
Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor 594 (Invitrogen, Carlsbad, USA)	1:200	1 h at RT
Chicken anti-Rabbit IgG Secondary Antibody, Alexa Fluor 488 (Invitrogen, Carlsbad, USA)	1:200	1 h at RT
Chicken anti-Rabbit IgG Secondary Antibody, Alexa Fluor 594 (Invitrogen, Carlsbad, USA)	1:200	1 h at RT
DAPI (Invitrogen, Carlsbad, USA)	1:5000	10 min RT

O.N.: overnight, RT stand for room temperature. DAPI is 4’,6-diamidin-2-fenilindolo.