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. 2025 Nov 7;13:1674916. doi: 10.3389/fbioe.2025.1674916

FIGURE 5.

Multiple panels are shown with bar graphs, a Western blot, and scatter plots depicting cell viability, LDH release, TNF-α, IL-6, MDA, SOD, GSH levels, protein expression of Bax, Bcl-2, cleaved caspase-3, and apoptosis rates. Each panel compares various treatments: Control, H₂O₂, H₂O₂ with sh-BRD4, sh-SIRT3, and 3-TYP, with statistical significance indicated by asterisks. The Western blot shows protein bands for Bax, Bcl-2, cleaved caspase-3, and β-actin. The scatter plots illustrate cell populations for different treatments, and a bar graph summarizes apoptosis rates.

SIRT3 inhibition reverses the protective effects of BRD4 inhibition on H2O2-challenged AEC-II cells. (A,B) The viability and LDH release of AEC-II cells from each group. (C,D) TNF-α and IL-6 levels in AEC-II cell supernatant from each group. (E–G) MDA, SOD, and GSH levels in AEC-II cells from each group. (H) Bax, Bcl-2, and cleaved-caspase 3 protein levels in AEC-II cells from each group. (I) The apoptosis of AEC-II cells from each group. Cell experiments were performed in triplicates (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.