2. NMR Sensitivity Per Unit Concentration (SensC) of Three Tyr Isotopologs Bearing Variable Types of Isotopic Enrichments .
| Tyr isotopolog | SensC of pulse-acquire 1H NMR (no hyperpolarization) (A) | SensC of 1D LC-photo-CIDNP NMR (13C RASPRINT) (B) | sensitivity enhancement(B/A) |
|---|---|---|---|
| Tyr-U–13C,15N | 0.21 ± 0.01 | 0.71 ± 0.05 | 3.4 ± 0.3 |
| Tyr-α-13C | 0.1535 ± 0.0001 | 1.01 ± 0.02 | 6.6 ± 0.1 |
| QISP Tyr (Fluorescein dye) | 0.41 ± 0.01 | 1.87 ± 0.04 | 4.5 ± 0.1 |
| QISP Tyr (ATTO Thio 12 dye) | 0.41 ± 0.01 | 4.4 ± 0.1 | 10.7 ± 0.3 |
a
All data are based on NMR measurements on the Tyr 1Hα resonance at 14.1 T (600 MHz). Unless otherwise stated, all experiments were performed on 1 μM (light) and 360–500 μM (dark or pulse-acquire) samples with a 50 ms and 1.5 s recycle delay respectively. Data are shown as avg ± SE for n = 2. See details in Methods.