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. 2025 Oct 13;36(11):2497–2506. doi: 10.1021/acs.bioconjchem.5c00459

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AMP induces ferroptosis in COAD cells. (A) Detection of mitochondrial membrane potential; (B) ROS content assay in HCT116 cells; (C) Kit to detect GSH content level in HCT116 cells; (D) Kit to detect MDA content level in HCT116 cells; (E) RT-qPCR to detect the expression level of ferroptosis-related genes; (F) Western Blot to detect the expression level of ferroptosis-related proteins.