Figure 3. Specc1lΔCCD2/ΔCCD2 display decreased SPECC1L and F-actin expression in the pericentriolar region.
A-B. Mouse embryonic fibroblasts (MEFs) from WT(A) and Specc1ΔCCD2/ΔCCD2 (B) were stained for SPECC1L (white), F-actin (red), acetylated-α-tubulin (green) and DAPI. C-D. Quantification of a 1x1x1 μm cubic volume of interest (VOI) for SPECC1L (C) and F-actin (D) showed a significant reduction for both in ΔCCD2 mutant cells (****<0.0001, * 0.0138, N=3, n=15 cilia, Scale bar represents 2μm, Error bars represent Mean ± standard deviation (SD) ).
