Table 1:

Primers and probes used in this study are listed in the 5’ to 3’ direction. The probes are labeled with 5’FAM/ZEN/3’IBFQ, and all were synthesized by Integrated DNA Technologies (IDT)

IAV RNA species	Probe	Primer (FW)	Primer (RV) (5’->3’)
WT-PB2	TATGCAAGGCTGCAATGGGACTGA	TTAGGCAGAACCCAACAGAAG	ACTGATGATCCGCTTGTTCTC
NP	TGCCACTGAAATCAGAGCATCCGT	AGGCACCAAACGGTCTTAC	AAGCAGAGAGCACCATTCTC
DI250	AGTACGCTGTTCTGAGGGGATTCC	CCCGCGAGATACTCACAAAA	GCTCAGTTCATTGATGCTTAGT
DI143	CAAGGACAAACTTTATGGAGTATGCTAATT	GCAGACAAGAGGATAACGGAA A	CCGTTTCCGTTTCATTACCAAC
DI289	TGAGCAAGGACAAAAACTGAAGACCCAGAT	GCAGACAAGAGGATAACGGAAA	TCTTTGCCCAGAATGAGGAATC
Universal MBTUni		ACGCGTGATCAGCAAAAGCAGG	ACGCGTGATCAGTAGAAACAAGG
PB2-UTR primers		AGCGAAAGCAGGTCAATTAT	AGTAGAAACAAGGTCGTTTTTAAAC
PB2-Internal primers		CTAAGAAATCTAATGTCGCAG	CGCTGTCTGGCTGTCAGTAA