TABLE 4.
Enzymatic activities of butyrate-producing strains harvested in exponential and stationary phases
| Species | Strain | Enzyme activity (μmol−1 mg of protein−1 min−1)a
|
|||||
|---|---|---|---|---|---|---|---|
| Acetate kinase
|
Butyrate kinase
|
Butyryl-CoA:acetate-CoA transferase
|
|||||
| E | S | E | S | E | S | ||
| Coprococcus sp. | L2-50 | 0.67 ± 0.13 | 0.15 ± 0.07 | 0.82 ± 0.39 | 0.11 ± 0.05 | 118.39 ± 5.02 | 10.8 ± 4.29 |
| F. prausnitzii | A2-165 | 0.05 ± 0.00 | 1.49 ± 0.11 | <0.01 | <0.01 | 18.64 ± 7.07 | 19.0 ± 0.0 |
| Roseburia sp. | A2-181 | 2.29 ± 0.21 | 2.85 ± 0.65 | <0.01 | <0.01 | 15.58 ± 5.98 | 2.79 ± 1.58 |
| Roseburia sp. | A2-183 | 2.03 ± 0.34 | 0.61 ± 0.08 | <0.01 | <0.01 | 38.95 ± 3.40 | 9.1 ± 0.27 |
| R. intestinalis | L1-82 | 1.82 ± 0.02 | 1.61 ± 0.63 | <0.01 | <0.01 | 25.50 ± 0.71 | 8.9 ± 1.17 |
| R. intestinalis | L1-952 | 2.32 ± 0.02 | 3.52 ± 0.83 | <0.01 | <0.01 | 17.45 ± 2.52 | 1.32 ± 0.0 |
| R. intestinalis | L1-8151 | 1.76 ± 0.02 | 1.31 ± 0.03 | <0.01 | <0.01 | 9.24 ± 1.48 | 2.05 ± 0.49 |
a
Data are the means of results of duplicated assays of two separate cultures. ± indicates the range of values. E, exponential phase; S, stationary phase.