TABLE 2.
16S rRNA gene-targeted primers
| Short namea | Full nameb | Annealing temp (°C) | Sequence 5′-3′ | Specificity | Reference |
|---|---|---|---|---|---|
| 616V | S-D-Bact-0008-a-S-18 | 52 | AGA GTT TGA TYM TGG CTC | Most Bacteria | 26 |
| 630R | S-D-Bact-1529-a-A-17 | 52 | CAK AAA GGA GGT GAT CC | Most Bacteria | 26 |
| 1492R | S-*-Proka-1492-a-A-19 | 52, 60c | GGY TAC CTT GTT ACG ACT T | Most Bacteria and Archaea | Modified from reference 27 |
| ARGLO36F | S-G-Arglo-0036-a-S-17 | 52 | CTA TCC GGC TGG GAC TA | Archaeoglobus spp. | This study |
| DSBAC355F | S-*-Dsb-0355-a-S-18 | 60 | CAG TGA GGA ATT TTG CGC | Most “Desulfobacterales” and “Syntrophobacterales” | 59 |
| DSM172F | S-G-Dsm-0172-a-S-19 | 56 | AAT ACC GGA TAG TCT GGC T | Desulfomicrobium spp. | This study |
| DSM1469R | S-G-Dsm-1469-a-A-18 | 56 | CAA TTA CCA GCC CTA CCG | Desulfomicrobium spp. | This study |
| DSN61F | S-*-Dsn-0061-a-S-17 | 52 | GTC GCA CGA GAA CAC CC | Desulfonema limicola, Desulfonema ishimotonii | This study |
| DSN+1201R | S-*-Dsn-1201-a-A-17 | 52 | GAC ATA AAG GCC ATG AG | Desulfonema spp. and other Bacteria | This study |
a
Short name used in the reference or in this study.
b
Name of 16S rRNA gene-targeted oligonucleotide primer based on the nomenclature of Alm et al. (1).
c
The annealing temperature was 52°C when the primer was used with forward primer 616V or ARGLO36F, and the annealing temperature was 60°C when the primer was used with forward primer DSBAC355F.