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. 2002 Oct;68(10):5170–5176. doi: 10.1128/AEM.68.10.5170-5176.2002

TABLE 2.

Detection of tester-specific DNA fragments in 2,4-DAPG-producing Pseudomonas spp. by spot-blot hybridization

SSH clone or promoter Bacterial strainsa
Q2-87 (B) CHA0 (A) Pf5 (A) STAD384 (C) Q2-1 (B) MVW4-2 (Q) Q2-2 (E) Q37-87 (E) QT1-6 (E) D27B1 (M) FFL1R22 (J) CV1-1 (H) FTAD1R36 (I) W4-4 (L) 7MA12 (O) MVW1-1 (P) HT5-1 (N) FFL1R18 (G) F113 (K) JMP6 (F) JMP7 (F) FTAD1R34 (D) OC4-1 (D) FFL1R9 (D) MVW1-2 (D) Q2-5 (D) W2-6 (D) Q128-87 (D) ATCC49054 (D) QT1-5 (D) Q8r1-96 (D) Frequency (%)b
3 + 0.03
5 + + + 0.10
6 + + + + + + + + 0.26
8 + + + 0.10
12 + 0.03
13 + + + + + + + + + + 0.32
18 + + + + + + + + + + + + + 0.42
25 + + + + + + + + + + + + + + + + + + + + + + + + + + 0.84
26 + 0.03
28 + + + + + + + + + + 0.32
32 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 0.94
36 + + + + + + + + + 0.29
41 + + + + + + + 0.23
45 + 0.03
53 + + + + + + + 0.23
58 + 0.03
61 + + + + + + + + + + + + 0.39
62 + + + + + + + + + + + + + + + 0.48
64 + + + + + + + 0.23
66 + + + + + + + + + + + + + + + + + + + + + + + + + + 0.84
74 + 0.03
80 + + 0.06
81 + + + + + + + + + + + + + + + + + 0.55
83 + + 0.06
85 + + + + + + + + + + + + + + + + + + + + + 0.68
93 + + + + + + + + + + + + + + + + + + 0.58
101 + + + + + + + + + + + 0.35
103 + + + + + + + + 0.26
127 + + + + + + + + + + + + + 0.42
132 + 0.03
133 + 0.03
164 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 0.97
Similarityc 0.00 0.13 0.13 0.16 0.16 0.16 0.25 0.25 0.25 0.25 0.22 0.25 0.19 0.19 0.19 0.19 0.22 0.25 0.34 0.38 0.31 0.44 0.38 0.41 0.47 0.47 0.34 0.63 0.59 0.69 1.00
a

Letters in parentheses correspond to BOX-PCR genotypes (18, 19). The majority of tested strains are described in references 18 and 19, except for Q2-87 (10), CHA0 (34), Pf5 (20), 7MA12 (15), Q128-87 (11), ATCC49054 (31), and Q8r1-96 (23).

b

Frequency values correspond to the percentage of test strains containing homologous sequences.

c

Similarity to Q8r1-96 was calculated using the simple matching coefficient.