TABLE 2.
Inducer | Induction ratios
|
||
---|---|---|---|
nahR/Psal | xylS2/Pm | nahR/Psal::xylS2/Pm | |
Salicylate | 24 | 13 | 235 |
Anthranilate | 22 | 11 | 285 |
2-Acetylsalicylate | 40 | 10 | 249 |
4-Chlorosalicylate | 33 | 1 | 191 |
5-Chlorosalicylate | 32 | 3 | 268 |
3,5-Dichlorosalicylate | 18 | 1 | 147 |
5-Methoxysalicylate | 3 | 46 | 183 |
Benzoate | 1 | 44 | 82 |
3-Methylbenzoate | 11 | 63 | 240 |
2-Methoxybenzoate | 33 | 10 | 160 |
3-Methylsalicylate | 17 | 46 | 218 |
4-Methylsalicylate | 21 | 7 | 203 |
5-Methylsalicylate | 30 | 19 | 257 |
For most inducers induction was performed by using the inducer at a concentration of 2 mM; the only exception was 3,5-dichlorosalicylate, which was used at a concentration of 1 mM to avoid growth inhibition. The results are means from at least three independent experiments. The standard deviations were less than 28%.