TABLE 5.
Nitrate production of cell extracts during NO· turnover
| Protein extracta | Nitrate (μM)b |
|---|---|
| Wild type | 17 ± 4 |
| Control | 18 ± 5 |
| FHPg | 19 ± 5 |
| VHb-Red | 15 ± 5 |
| FHP | 19 ± 6 |
| VHb | 17 ± 4 |
| CHb | 16 ± 3 |
| HMP | 22 ± 1 |
| HmpSt | 23 ± 3 |
| HmpKp | 18 ± 4 |
| HmpBs | 24 ± 4 |
| HmpPa | 24 ± 7 |
| HmpDr | 26 ± 3 |
a
Soluble protein fractions were obtained from E. coli MG1655 cells overexpressing either hemoglobins or flavohemoglobins. The control strain contained the parental plasmid pKQV4.
b
Nitrate recovery after conversion of 20 μM NO· by cell extracts. Means from two independent assays and standard deviations are given. Each measurement has been done twice.