TABLE 2.
Specificity of conventional multiplex PCR assay for detection of the stx1 and stx2 genes of STEC and the eae gene of E. coli O157:H7
| Bacterial strain type tested | Strain no. | Serotype | Genotypea
|
Origin/disease or status | ||
|---|---|---|---|---|---|---|
| stx1 | stx2 | eae | ||||
| Nontoxigenic E. coli | 63 | O78:H16 | − | − | − | Dog |
| 912 | OX3:H11 | − | − | − | Pig/normal | |
| EPEC | 1861 | O119 | − | − | − | Human/diarrhea |
| 1987 | O111:H2 | − | − | − | Human | |
| 1988 | O26:NM | − | − | − | Human | |
| 5722 | O55:NM | − | − | + | Human | |
| ETEC | 431 | O101 | − | − | − | Pig/diarrhea |
| 1477 | O149:H10 | − | − | − | Pig/diarrhea | |
| STEC | 5702 | O103:H2 | + | − | + | Human/HUS |
| 5354 | O157:H7 | + | − | + | Calf/normal | |
| 3048 | O157:NM | + | + | + | Calf | |
| 5264 | O157:NM | + | + | + | Calf/normal | |
| 4178 | O157:H7 | + | + | + | Human/HUS | |
| 5705 | O121:H19 | + | + | − | Human/HUS | |
| 3883 | O111:NH | + | − | + | Calf/normal | |
| 5710 | O26:H11 | + | + | + | Human/HUS | |
| 2871 | O157:H7 | − | + | + | Calf/normal | |
| 2725 | O157:H7 | + | + | + | HUS | |
| 5570 | O157:H7 | + | + | + | Human/diarrhea | |
| 3128 | O113 | + | + | − | Human/diarrhea | |
| 2799 | O157:NH | − | + | + | Calf/normal | |
| 5709 | O111:NM | + | + | + | Human/HUS | |
| 5981 | O157:NM | − | − | + | Calf | |
| 5701 | O126:H27 | + | − | − | Human/HUS | |
| 3861 | OX3:H2 | + | + | + | Calf/normal | |
| 2409 | O157:H7 | + | − | + | Food | |
| 1043 | O22 | + | − | + | Calf/diarrhea | |
| 2873 | O157:NM | + | + | + | Calf/normal | |
| 5708 | O45:H2 | + | − | − | Human/HUS | |
| 4700 | O157:H7 | + | + | + | Calf | |
| 3081 | O157:H7 | + | + | + | Calf | |
| 1524 | O132 | − | − | + | Rabbit/diarrhea | |
| 3244 | O119:H16 | − | + | − | Calf/normal | |
a
The presence of these genes had been determined by Sharma et al. (28) by fluorogenic PCR with DNA probes specific for these genes and confirmed in this study. +, gene detected by multiplex PCR; −, gene not detected by multiplex PCR.