TABLE 3.
Sensitivity of real-time multiplex PCR assay for detection and quantification of the stx1 and the stx2 genes of STEC, eae of E. coli O157:H7, and related strains
| Bacterial strain type | Strain no. | Serotype | Genotype |
CT of gene:
|
||
|---|---|---|---|---|---|---|
| stx1 | stx2 | eae | ||||
| Nontoxigenic E. coli | 63 | O78:H16 | 42.3 | −a | − | |
| Salmonella enterica serovar Typhimurium | 3244 | O119:H16 | 46.9 | − | − | |
| EPEC | 5722 | O55:NM | eae | 38.1 | 47.7 | 43.8 |
| ETEC | 431 | O101 | stap | 38.8 | − | − |
| STEC | 5701 | O116:H27 | stx1 | 24.6 | − | 47.8 |
| 4718 | O157:H7 | stx1 stx2 eae | 24.8 | 29.2 | 26.0 | |
| 4700 | O157:H7 | stx1 stx2 eae | 25.1 | 31.1 | 27.6 | |
| 3081 | O157:H7 | stx1 stx2 eae | 23.9 | 28.4 | 26.1 | |
| 5699 | O91:H21 | stx2 | 39.2 | 26.3 | − | |
a
−, CT not determined by real-time PCR.