FIG. 2.
(a) Map of cosmid SC5A7 containing a cluster of PPP-related genes. The open arrows indicate the directions and extents of the predicted protein-encoding regions. Genes encoding predicted proteins showing strong homology to proteins having known metabolic functions are indicated by boldface italic type. Genes encoding predicted proteins having unknown functions are designated hyp. Segments of DNA that were used to make the in-frame zwf1 deletion are indicated by lines terminating in solid circles. The extent and location of the in-frame deletion in the deletion plasmid pIJ8722 and the Δzwf1 deletion strain M704 are indicated by a dotted line. The fragments (from pIJ8707 and pIJ8719) used to produce pIJ8715 are indicated by shaded boxes. The region of the zwf1 cluster cloned in pIJ8715 and used to complement the Δzwf1 mutation is indicated by a line terminating in solid diamonds. Naturally occurring restriction enzyme sites are indicated by boldface type, while sites introduced by PCR or from vector polylinkers are indicated by lightface type in parentheses. (b) Map of cosmid StC22 containing a second cluster of PPP-related genes. The restriction enzyme sites used to join the various fragments were added by using PCR primers. The box labeled hygR shows the position of the insertion of the hygromycin resistance gene in the ΔdevB mutant strain. The extents of deleted DNA in the deletion plasmids and strains are indicated by dotted lines.