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Physical map of Bcmfs1 in genomic DNA from B. cinerea wild-type strain B05.10 (A) and replacement mutant of Bcmfs1 with the hygromycin-resistance (OHT) cassette (B) or the nourseothricin-resistance (ONT) cassette (C). Southern blots of DNA from single-replacement mutants and double-replacement mutants of Bcmfs1 were hybridized with the 5.5-kb XbaI-SalI fragment and the 3.6-kb XbaI-ApaI fragment, respectively. Northern blots were hybridized with the EST fragment of Bcmfs1.
