FIG. 2.

Southern blot analysis of DNA from B. cinerea. Genomic DNA (5 μg) was digested with EcoRV and hybridized with a 5.5-kb XbaI-SalI probe (A) and with a 3.6-kb XbaI-ApaI probe (B). Results obtained with parental strain B05.10, Bcmfs1 replacement mutants ΔBcmfs1-16 and ΔBcmfs1-18, reference mutant HR-9, BcatrD replacement mutant ΔBcatrD-8, BcatrB and Bcmfs1 double-replacement mutant ΔBΔ1-22, BcatrD and Bcmfs1 double-replacement mutant ΔDΔ1-45, reference mutant HNR-4, and BcatrB replacement mutant ΔBcatrB4 are shown.