Skip to main content
. 2002 Oct;68(10):4996–5004. doi: 10.1128/AEM.68.10.4996-5004.2002

FIG. 3.

FIG. 3.

Northern blot analysis of total RNA (10 μg) from germlings of B. cinerea. (A) Parental strain B05.10, Bcmfs1 replacement mutants ΔBcmfs1-16 and ΔBcmfs1-18, reference mutant HR-9, and BcatrD replacement mutant ΔBcatrD-8. Basal levels of expression (lanes 1) and expression levels after treatment with 30 mg of oxpoconazole liter−1 (lanes 2) are shown. (B) Parental strain B05.10, BcatrB and Bcmfs1 double-replacement mutant ΔBΔ1-22, BcatrD and Bcmfs1 double-replacement mutant ΔDΔ1-45, reference mutant HNR-4, BcatrB replacement mutant ΔBcatrB4, BcatrD replacement mutant ΔBcatrD-8, and Bcmfs1 replacement mutant ΔBcmfs1-16. Basal levels of expression (lanes 1) and expression levels after treatment with 30 mg of oxpoconazole liter−1 (lanes 2) are shown. (C) Parental strain B05.10 (lanes 1) and the Bcmfs1 overexpression mutants OV1-23 (lanes 2), OV1-48 (lanes 3), and OV1-13 (lanes 4). Basal and induced expression levels after treatment with 3, 10, and 30 mg of oxpoconazole liter−1 are shown. RNA was hybridized with the EST probe specific for Bcmfs1 (Fig. 1A). Equal loading of lanes with RNA was checked by subsequently probing the same blot with 28S rRNA.