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. 2002 Oct;68(10):5136–5141. doi: 10.1128/AEM.68.10.5136-5141.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — (A) Expression plasmid for display of T. reesei EGII (pEG23u31H6) on the yeast cell surface. (B) Immunofluorescence labeling of transformed cells: Nomarski differential interference micrographs (column 1) and immunofluorescence micrographs (columns 2 and 3) of S. cerevisiae MT8-1/pMUCS (control), MT8-1/pEG23u31H6, MT8-1/pBG211 and MT8-1/pBG211/pEG23u31H6. Cells were labeled with mouse anti-RGSHis4 antibody and goat anti-mouse IgG conjugated with Alexa Fluor 546 and with rabbit anti-A. aculeatus BGL1 antibody and goat anti-rabbit IgG conjugated with FITC.