TABLE 1.

Strains and plasmids

Bacterium or plasmid	Characteristic(s)	Reference or source
L. lactis
MG1363		10
MG1614		10
MG1614Δthr	Δhom ΔthrB	27
MG1363Δthr	Δhom ΔthrB	This work
E. coli DH10B	Laboratory strain	Invitrogen
Plasmids
pGEM7Zf(+)	lacZ bla	Promega, Southampton, United Kingdom
pCR2.1	lacZ bla kan	Invitrogen
pEGFP-N1	CMV promoter-EGFP-SV40 poly(A); kan	Clontech, Palo Alto, Calif.
pG3E	pGEM-3Zf(−)::1.2 kb; ermL	32
pSMA231	pGEM-7Zf(+)::1.8 kb; Δhom-thrB	27
pSMA507	Δhom ΔthrB lacLM ermL	27
pSMBI109	Citrate replicon-P170 promoter-SP310mut2; nucB ermL	A. Vrang, unpublished
pAK80	Citrate replicon	16
pJAG1	pCR2.1::0.6 kb; 5′ end of hom	This work
pJAG2	pGEM-7Zf(+)::2.4 kb; hom-thrB	This work
pJAG3	pG3E::1.8 kb; citrate replicon	This work
pJAG4	pJAG3::2.4 kb; hom-thrB	This work
pJAG5	Citrate replicon; hom-thrB	This work
pJAG6	pJAG5::0.9 kb; P170 promoter-SP310mut2; nucB	This work
pJAG7	pCR2.1::1.6 kb; CMV promoter-EGFP-SV40 pA	This work
pJAG8	pJAG4::1.6 kb; CMV promoter-EGFP-SV40 pA	This work