FIG. 5.

Analysis of conformation of OmpF and OmpF-β-endorphin fusion protein excreted into the culture medium. Lanes: 1, molecular mass standards; 2 and 3, culture supernatant from the high-cell-density culture of E. coli BL21(DE3); 4 and 5, culture supernatant from the high-cell-density culture of E. coli MBEL-BL101 harboring pOmpF6βE. Lanes 2 and 4 show the boiled samples, and lanes 3 and 5 show the unboiled samples. The solid arrows indicate the monomeric form of OmpF and OmpF fusion proteins, and the dashed arrow indicates the trimeric form of OmpF and OmpF fusion proteins.