(A) Slot blot hybridization of gDNAs from DPRB and non-perchlorate-reducing close relatives using the D. agitata probe to the 3′ end of the CD gene. Organisms capable of chlorate or perchlorate reduction are underlined. Row A (left to right): lane 1, D. agitata; lane 2, Rhodocyclus tenuis; lane 3, Dechloromonas aromatica; lane 4, Dechloromonas sp. strain JJ. Row B: lane 1, Dechlorospirillum anomalous strain WD; lane 2, Magnetospirillum magnetotacticum; lane 3, Pseudomonas sp. strain PK; lane 4, Pseudomonas stutzeri. Row C: lane 1, Dechloromarinus sp. strain NSS; lane 2, Dechlorosoma suillum; lane 3, Azoarcus sp. strain LT-1; lane 4, I. dechloratans. (B) Phylogenetic tree of the organisms used in the slot blot hybridization based on 16S rDNA sequences (1, 9).