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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 1. TRIM7 expression is increased in the development and progression of NAFLD.

Fig. 1

a TRIM7 gene expression data from RNA-Seq analysis of liver tissues from the indicated mouse models. b, c RT-qPCR analysis of relative TRIM7 mRNA expression in liver tissues of ob/ob mice (12 weeks) and C57BL6/J mice fed with HFD for 12 weeks (NCD, HFD group n = 8; ob/ob group n = 6; P values determined by one-way ANOVA) (b) or HFFC fed for 19 weeks (n = 10 per group; P values determined by 2 tailed t test) (c). d, e Western Blot (d) and quantitative analysis (e) of TRIM7 protein expression in liver tissues (ob/ob group n = 3; NCD, HFD, HFFC group n = 4; P < 0.001 versus NCD group by 2 tailed t test). f Representative immunohistochemistry (IHC) staining images of TRIM7 in liver tissues from mice fed NCD, HFD, or HFFC diet (n = 3). g, h Analysis of TRIM7 protein expression in liver tissues of mice fed a HFD and HFFC diet for different time (n = 3/group, P values determined by one-way ANOVA). i, j TRIM7 protein expression analysis in liver tissues from non-steatosis and NAFLD patients (non-steatosis group n = 6; NAFLD group n = 8; P < 0.001 by 2 tailed t test). k Representative immunohistochemistry staining images of TRIM7 in liver tissues from non-steatosis and NAFLD patients (n = 3). l Representative immunofluorescence staining images of TRIM7 and HNF4 in liver tissues from HFD-fed mice for 12 weeks (n = 3). m, n Western Blot (m) and quantitative analysis (n) of TRIM7 protein expression in murine primary hepatocytes treated with POA or BSA (n = 4; P < 0.001 versus BSA group by 2 tailed t test). Data are presented as mean ± SD. Source data are provided as a Source Data file.