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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 2. Hepatocyte-specific TRIM7 deficiency ameliorates HFD-induced metabolic disturbance and liver injury.

Fig. 2

a–d Body weight (a), fasting blood glucose levels (b), fasting insulin levels (c), and HOMA-IR (d) in Trim7-HKO and Trim7-Flox mice at the indicated time points during feeding with NCD or HFD (n = 8; P values determined by one-way ANOVA). e, f The IPGTT (e) and IPITT (f) were performed in Trim7-HKO and Trim7-Flox mice at 12 weeks and 13 weeks of NCD or HFD feeding, respectively, and the areas under the curve (AUC) were calculated for both tests (n = 8; P values determined by one-way ANOVA); *P < 0.05, **P < 0.01, ***P < 0.001 vs. NCD-Trim7-Flox group; #P < 0.05, ##P < 0.01 vs. HFD-Trim7-Flox group. g Immunoblotting analysis of the phosphorylated and total expression levels of the indicated proteins in the insulin signaling pathway within liver tissues (n = 3). h Representative PAS staining images of liver tissues from Trim7-HKO and Trim7-Flox mice fed a NCD or HFD for 14 weeks (n = 4). i, j The liver weight (i), liver weight to body weight ratio (j) in Trim7-HKO and Trim7-Flox mice fed a NCD or HFD for 14 weeks (n = 8; P values determined by one-way ANOVA). k, l H&E and Oil red O staining (k), quantitative analysis of Oil red O positive area (n = 5; P < 0.001 by 2 tailed t test) (l) in liver tissues from Trim7-HKO and Trim7-Flox mice fed a NCD or HFD for 14 weeks. m–o Serum levels of TNFα (m), IL-6 (n) and IL-10 (o) in Trim7-HKO and Trim7-Flox mice fed a NCD or HFD for 14 weeks (n = 8; P values determined by one-way ANOVA). p Immunofluorescence staining for F4/80 (macrophage marker) in liver sections from HFD-fed Trim7-HKO and Trim7-Flox mice (n = 6). Data are presented as mean ± SD. Source data are provided as a Source Data file.