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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 3. TRIM7-HKO alleviates HFFC-induced NASH.

Fig. 3

a–f Body weight (a), liver weight (b), liver weight-to-body weight ratio (c), fasting blood glucose levels (d), fasting insulin levels (e) and HOMA-IR (f) in Trim7-HKO and Trim7-Flox mice at the indicated time points during feeding with NCD or HFFC (n = 8; a performed by 2 tailed t test, b-f performed by one-way ANOVA). g, h The IPGTT (g) and IPITT (h) were performed at 17 weeks and 18 weeks of HFFC feeding, respectively, and the areas under the curve (AUC) were calculated for both tests (n = 8; P values determined by 2 tailed t test). i Immunoblotting analysis of phosphorylated and total expression levels of the indicated proteins in the insulin signaling pathway in liver tissues (n = 3). j–m Representative PAS staining images of the liver (j), liver H&E and Oil red O staining images (k), hepatic contents of TG, TC and NEFA (l), and serum levels of TNFα, IL-6 and IL-10 (m) in Trim7-HKO and Trim7-Flox mice fed a NCD or HFFC diet for 19 weeks (n = 8; P values determined by one-way ANOVA). n, o Representative immunofluorescence staining images for F4/80 (n) and Sirius Red staining images (o) in liver sections from Trim7-HKO and Trim7-Flox mice fed a HFFC diet (n = 4). Data are presented as mean ± SD. Source data are provided as a Source Data file.