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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 4. Hepatic TRIM7 overexpression exacerbates HFD-induced steatohepatitis.

Fig. 4

a Schematic of the AAV8-mediated Trim7 overexpression model in NCD- or HFD-fed mice. b Representative immunohistochemistry staining images of Trim7 in the liver of AAV8-injected mice (n = 5). c–h Body weight (c), liver weight (d), liver weight-to-body weight ratio (e), fasting blood glucose levels (f), fasting insulin levels (g), and HOMA-IR (h) in AAV8-injected mice fed a NCD or HFD for 14 weeks (n = 5; P values determined by one-way ANOVA). i–j The IPGTT (i) and IPITT (j) were performed at 12 weeks and 13 weeks of HFD feeding, respectively, and the areas under the curve (AUC) were calculated for both tests (n = 5; P values determined by one-way ANOVA). k–m Immunoblotting analysis of the indicated protein expression (k), H&E and Oil red O staining images (l), and quantitative analysis of Oil red O positive area (m) in liver tissues of AAV8-injected mice fed a HFD for 14 weeks (n = 5; P values determined by one-way ANOVA). n, o Serum levels of TNFα, IL-6 (n) and IL-10 (o) in AAV8-injected mice fed a NCD or HFD for 14 weeks (n = 10; P values determined by one-way ANOVA). p Immunofluorescence staining for F4/80 in liver sections of AAV8-injected mice fed a HFD for 14 weeks (n = 5). Data are presented as mean ± SD. Source data are provided as a Source Data file.