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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 5. Hepatic TRIM7 overexpression exacerbates HFFC-induced steatohepatitis.

Fig. 5

a Schematic illustration of the AAV8-mediated Trim7 overexpression model in HFFC-fed mice. b Representative immunohistochemistry staining images of Trim7 in the liver of AAV8-injected mice (n = 5). c–h Body weight (c), liver weight (d), liver weight-to-body weight ratio (e), fasting blood glucose levels (f), fasting insulin levels (g) and HOMA-IR (h) in AAV8-injected mice fed a HFFC diet for the indicated time (n = 5; P values determined by one-way ANOVA). i, j The IPGTT (i) and IPITT (j) were performed after 17 weeks and 18 weeks of HFFC diet feeding, respectively, and the areas under the curve (AUC) were calculated for both assays (n = 5; P values determined by one-way ANOVA). k–m Immunoblotting analysis of the indicated protein expression in liver tissues (k), H&E staining and Oil red O staining images of liver sections (l), and quantitative analysis of Oil Red O positive areas in liver tissues (m) in AAV8-injected mice fed a HFFC diet for 19 weeks (n = 5; P values determined by one-way ANOVA). n–q Serum levels of IL-10 (n) and TNFα, IL-6 (o) (n = 10; P values determined by one-way ANOVA); liver immunofluorescence staining images for F4/80 (p), and Sirius Red staining images (q) in AAV8-injected mice fed a HFFC diet for 19 weeks. Data are presented as mean ± SD. Source data are provided as a Source Data file.