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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — a Volcano plot showing the distribution of all detected genes from RNA sequencing in liver tissues of Trim7-HKO and Trim7-Flox mice after 14 weeks of HFD feeding (n = 3). Differentially expressed genes (DEGs) analysis was performed using the two-sided Wald test, which was selected to account for both potential upregulation and downregulation of genes between the two groups. Genes with a P-value < 0.05 and |log2 (fold change)|≥1.0 were defined as DEGs; red dots represent significantly upregulated DEGs, blue dots represent significantly downregulated DEGs, and black dots represent non-significant genes. b KEGG pathway enrichment analysis of the aforementioned DEGs. c, d Immunoblotting (c) and quantitative (d) analysis of the expression levels of the indicated proteins in liver tissues from NCD- or HFFC-fed Trim7-Flox, Trim7-HKO mice (left panel) and AAV8-injected mice (right panel) (n = 3; P values determined by one-way ANOVA). Data are presented as mean ± SD. Source data are provided as a Source Data file.