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. 2025 Nov 25;16:10437. doi: 10.1038/s41467-025-65415-6

Fig. 8. DUSP10 is essential for TRIM7-mediated function in steatohepatitis.

Fig. 8

a Schematic illustration of the AAV8-mediated DUSP10 downregulation in the liver of HFD-fed Trim7-HKO mice. b, c Immunoblotting analysis (b) and quantitative (c) analysis of DUSP10 expression in liver tissues from the above AAV8-injected mice (n = 4; P values determined by 2 tailed t test). d–h Liver weight (d), liver weight/body weight ratio (e), fasting blood glucose levels (f), fasting insulin levels (g), and HOMA-IR (h) in the above AAV8-injected mice after 14 weeks of HFD feeding (n = 8; P values determined by 2 tailed t test). i, j The IPGTT (i) and IPITT (j) were conducted on the above AAV8-injected mice at 12 weeks and 13 weeks of HFD feeding, respectively. Subsequently, the areas under the curve (AUC) for each test were calculated (n = 8; P values determined by 2 tailed t test). k Immunoblotting analysis of the indicated proteins expression in liver tissues from the above AAV8-injected mice after 14 weeks of HFD feeding (n = 4). l–n Liver PAS staining images (l), liver H&E and Oil red O staining images (m), and quantitative analysis of Oil Red O-positive areas in liver tissues (n = 5; P values determined by 2 tailed t test). o ELISA analysis of the serum levels of TNFα, IL-6, and IL-10 in the above AAV8-injected mice after 14 weeks of HFD feeding (n = 8; P values determined by 2 tailed t test). p Immunofluorescence staining images for F4/80 in liver tissues from the above AAV8-injected mice (n = 6). q Immunoblotting analysis of the indicated protein expression in liver tissues from the above AAV8-injected mice (n = 4). Data are presented as mean ± SD. Source data are provided as a Source Data file.