| 2018 |
ilux (enhanced Lux operon) |
bacteria |
∼7×
brighter lux operon, improved substrate flux |
demonstrated long-term autonomous
imaging in bacteria |
|
| 2018 |
FBP1 (fungal bioluminescence pathway 1) |
yeast and mammalian
cells |
full pathway of fungal bioluminescence system |
enable long-term autonomous
imaging in yeast with FBP1 |
|
| 2019 |
co Lux |
mammalian cells |
codon-optimized Lux and Frp
expression |
single mammalian cells autonomous bioluminescence
imaging |
|
| 2021 |
BRET-Lux fusions |
bacteria, mammalian, and plant |
fusion of luciferase with fluorescent acceptor (Venus) to shift
emission and boost light |
enhanced spectral flexibility
and brightness via energy transfer |
|
| 2022 |
Cryo-EM of LuxC–LuxE |
bacterial enzymes |
structural elucidation of fatty acid reductase complex |
informed rational protein engineering |
|
| 2022 |
ilux2
|
bacteria |
integration of Lux mutants into chromosomes
for stable expression
and brightness gains |
enables durable and bright autobioluminescent
bacterial strains
for long-term use |
|
| 2020 |
plant with FBP1 |
plant |
engineered fungal bioluminescence pathway in plant expression |
self-sustained luminescence
plant that is visible to the naked eye |
,
|
| 2023 |
eFBP (enhanced fungal bioluminescence pathway) |
plant |
metabolic engineering of fungal luciferin biosynthesis by introducing
coumaroyl shikimate/quinate 30-hydroxylase (C3′H) gene |
enhanced
brightness of luminescent plants up to 3 × 1011 photons/min/cm2
|
|
| 2023 |
FBP2/3 (optimized fungal bioluminescence
pathway) |
yeast, plant, and mammalian cells |
engineered fungal pathway with much higher brightness |
stronger autoluminescence across diverse hosts |
|
| 2024 |
A hybrid FBP |
yeast, plant, and mammalian
cells |
A hybrid pathway of FBP with new hispidin biosynthesis
genes
(type III polyketide synthases) |
reduce the size of HispS
gene (5.1 kbp) to 1.2 kbp (PpASCL) |
|
| 2024 |
nano-lanternX (NLX) (multicolor
Lux) |
bacteria, mammalian, and plant |
designed
5 spectrally distinct autonomous reporters via BRET-Lux |
multiplexed, substrate-free spectral
imaging across diverse hosts |
|
| 2025 |
transgenic autobioluminescent mouse |
mammalian whole
organism |
chromosomal insertion of full bacterial lux
genes enabling in vivo autonomous bioluminescence |
first mammal with substrate-free bioluminescence for animal imaging |
|
