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. 1966 Mar;98(3):782–786. doi: 10.1042/bj0980782

The isolation and partial characterization of glycolipids of normal human leucocytes

Constantinos J Miras 1, John D Mantzos 1, Gabriel M Levis 1
PMCID: PMC1264919  PMID: 5911526

Abstract

1. The lipids of purified human leucocytes were extracted with chloroform–methanol and the extract was washed with water. Glycolipids, isolated by Florisil chromatography, were subjected to mild alkaline hydrolysis and the alkali-resistant fraction was fractionated on a silicic acid column. 2. Three classes of glycolipid were separated. The less polar, containing 3·6% of the total glycolipid hexose as galactose, was tentatively identified as ceramide monohexoside. The major glycolipid fraction was characterized as ceramide dihexosides. The more polar glycolipids comprised 1·6% of the total glycolipid hexose as galactose and glucose (in the molar ratio 2:1) and were non-acidic. This class was separated as a mixture containing ninhydrin-positive glycolipids. 3. The ceramide dihexosides taken from two leucocyte preparations accounted for 15·2% and 16·4% by weight of the total lipids. 4. The carbohydrate moiety of the ceramide dihexosides contained galactose and glucose in the molar ratio 2:1. Partial acid hydrolysis and paper chromatography indicated that the hexoses are present as disaccharides, lactose being identified as one of them. 5. Palmitic acid (C16:0) and nervonic acid (C24:1) were the major fatty acids of this glycolipid. Hydroxy fatty acids were not detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  2. FUJINO Y., ZABIN I. The configuration of sphingosine synthesized in rat brain homogenates. J Biol Chem. 1962 Jul;237:2069–2071. [PubMed] [Google Scholar]
  3. GRAY G. M. THE ISOLATION AND PARTIAL CHARACTERIZATION OF THE GLYCOLIPIDS OF BP8/C3H ASCITES-SARCOMA CELLS. Biochem J. 1965 Jan;94:91–98. doi: 10.1042/bj0940091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GRAY G. M. The lipid composition of tumour cells. Biochem J. 1963 Feb;86:350–357. doi: 10.1042/bj0860350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAKOMORI S. I., JEANLOZ R. W. Isolation and characterization of glycolipids from erythrocytes of human blood A (plus) and B (plus). J Biol Chem. 1961 Nov;236:2827–2834. [PubMed] [Google Scholar]
  6. HUBSCHER G., HAWTHORNE J. N., KEMP P. The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver. J Lipid Res. 1960 Oct;1:433–438. [PubMed] [Google Scholar]
  7. JATZKEWITZ H. [The sulfuric acid esters of cerebron and kerasin as storage substances in leukodystrophy of the Scholz type (metachromatic form of diffuse sclerosis)]. Hoppe Seylers Z Physiol Chem. 1960 Aug 31;320:134–148. doi: 10.1515/bchm2.1960.320.1.134. [DOI] [PubMed] [Google Scholar]
  8. King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LLOYD A. G., DODGSON K. S. Infrared studies on sulphate esters. II. Monosaccharide sulphates. Biochim Biophys Acta. 1961 Jan 1;46:116–120. doi: 10.1016/0006-3002(61)90653-9. [DOI] [PubMed] [Google Scholar]
  10. MEAD J. F., LEVIS G. M. A 1 carbon degradation of the long chain fatty acids of brain sphingolipids. J Biol Chem. 1963 May;238:1634–1636. [PubMed] [Google Scholar]
  11. RADIN N. S., BROWN J. R., LAVIN F. B. The preparative isolation of cerebrosides. J Biol Chem. 1956 Apr;219(2):977–983. [PubMed] [Google Scholar]
  12. RADIN N. S. Glycolipide chromatography. Fed Proc. 1957 Sep;16(3):825–826. [PubMed] [Google Scholar]
  13. RADIN N. S., LAVIN F. B., BROWN J. R. Determination of cerebrosides. J Biol Chem. 1955 Dec;217(2):789–796. [PubMed] [Google Scholar]
  14. RAPPORT M. M., GRAF L., SKIPSKI V. P., ALONZO N. F. Immunochemical studies of organ and tumor lipids. VI. Isolation and properties of cytolipin H. Cancer. 1959 May-Jun;12(3):438–445. doi: 10.1002/1097-0142(195905/06)12:3<438::aid-cncr2820120303>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  15. RAPPORT M. M., GRAF L., YARIV J. Immunochemical studies of organ and tumor lipids. IX. Configuration of the carbohydrate residues in cytolipin H. Arch Biochem Biophys. 1961 Mar;92:438–440. doi: 10.1016/0003-9861(61)90382-4. [DOI] [PubMed] [Google Scholar]
  16. RAPPORT M. M., SKIPSKI V. P., SWEELEY C. C. The lipid residues in cytolipin H. J Lipid Res. 1961 Apr;2:148–151. [PubMed] [Google Scholar]
  17. SVENNERHOLM E., SVENNERHOLM L. THE SEPARATION OF NEUTRAL BLOOD-SERUM GLYCOLIPIDS BY THIN-LAYER CHROMATOGRAPHY. Biochim Biophys Acta. 1963 Aug 27;70:432–441. doi: 10.1016/0006-3002(63)90773-x. [DOI] [PubMed] [Google Scholar]
  18. SWEELEY C. C. PURIFICATION AND PARTIAL CHARACTERIZATION OF SPHINGOMYELIN FROM HUMAN PLASMA. J Lipid Res. 1963 Oct;4:402–406. [PubMed] [Google Scholar]
  19. YAMAKAWA T., KISO N., HANDA S., MAKITA A., YOKOYAMA S. On the structure of brain cerebroside sulfuric ester and ceramide dihexoside of erythrocytes. J Biochem. 1962 Sep;52:226–227. doi: 10.1093/oxfordjournals.jbchem.a127602. [DOI] [PubMed] [Google Scholar]

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