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. 1966 Aug;100(2):448–457. doi: 10.1042/bj1000448

Effects of colchicine on nucleic acid metabolism during metamorphosis of Tenebrio molitor L. and in some mammalian tissues

J Ilan 1,*, J H Quastel 1
PMCID: PMC1265155  PMID: 5968543

Abstract

1. Administration of 10μg. of colchicine/pupa of the beetle Tenebrio molitor L. arrests its differentiation, the pupa remaining alive for 2–3 weeks. 2. The same concentration of colchicine inhibits DNA synthesis and stimulates RNA synthesis (as shown by incorporation into the nucleic acids of labelled adenine, labelled uridine and labelled thymidine). The effects of colchicine on nucleic acid metabolism are first detected 3 days after its administration to first-day pupae. 3. No effects of colchicine are seen on [1-14C]glycine incorporation into protein in vivo. 4. Relatively high concentrations of colchicine (e.g. 10mm) suppress incorporation of [8-14C]adenine into RNA in dorsal abdominal wall in vitro. Such concentrations have no effect on its incorporation into acid-soluble nucleotides. 5. Colchicine (1mm) suppresses incorporation of [8-14C]adenine into DNA to a greater extent than into RNA in various mammalian tissues in vitro (e.g. rat spleen, regenerating rat liver, rat embryo, guinea-pig intestinal mucosa, Ehrlich ascites cells). Colchicine (1mm) has no effect on the rate of respiration of, or on incorporation of radioactivity into acid-soluble nucleotides in, the mammalian tissues tested. 6. Further evidence indicates complex-formation between colchicine and DNA, and it is suggested that the effect of colchicine in suppressing DNA synthesis is due to its combination with the DNA primer (template).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENITEZ H. H., MURRAY M. R., CHARGAFF E. Studies on inhibition of the colchicine effect on mitosis. Ann N Y Acad Sci. 1954 Nov 17;58(7):1288–1302. doi: 10.1111/j.1749-6632.1954.tb45909.x. [DOI] [PubMed] [Google Scholar]
  2. CREASEY W. A., MARKIW M. E. BIOCHEMICAL EFFECTS OF THE VINCA ALKALOIDS. II. A COMPARISON OF THE EFFECTS OF COLCHICINE, VINBLASTINE AND VINCRISTINE ON THE SYNTHESIS OF RIBONUCLEIC ACIDS IN EHRLICH ASCITES CARCINOMA CELLS. Biochim Biophys Acta. 1964 Aug 12;87:601–609. doi: 10.1016/0926-6550(64)90278-6. [DOI] [PubMed] [Google Scholar]
  3. GRANT P. The influence of folic acid analogs on development and nucleic acid metabolism in Rana pipiens embryos. Dev Biol. 1960 Jun;2:197–251. doi: 10.1016/0012-1606(60)90007-5. [DOI] [PubMed] [Google Scholar]
  4. GROSS P. R., COUSINEAU G. H. Effects of actinomycin D on macromolecule synthesis and early development in sea urchin eggs. Biochem Biophys Res Commun. 1963 Feb 18;10:321–326. doi: 10.1016/0006-291x(63)90532-1. [DOI] [PubMed] [Google Scholar]
  5. HELL E., COX D. G. Effects of colchicine and colchemid on synthesis of deoxyribonucleic acid in the skin of the guinea pig's ear in vitro. Nature. 1963 Jan 19;197:287–288. doi: 10.1038/197287a0. [DOI] [PubMed] [Google Scholar]
  6. IYER V. N., SZYBALSKI W. MITOMYCINS AND PORFIROMYCIN: CHEMICAL MECHANISM OF ACTIVATION AND CROSS-LINKING OF DNA. Science. 1964 Jul 3;145(3627):55–58. doi: 10.1126/science.145.3627.55. [DOI] [PubMed] [Google Scholar]
  7. Ilan J., Quastel J. H. Effects of actinomycin D on nucleic acid metabolism and protein biosynthesis during metamorphosis of Tenebrio molitor L. Biochem J. 1966 Aug;100(2):441–447. doi: 10.1042/bj1000441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LETTRE H. Some investigations on cell behavior under various conditions; a review. Cancer Res. 1952 Dec;12(12):847–860. [PubMed] [Google Scholar]
  9. NYGAARD O. F., GUTTES S., RUSCH H. P. Nucleic acid metabolism in a slime mold with synchronous mitosis. Biochim Biophys Acta. 1960 Feb 26;38:298–306. doi: 10.1016/0006-3002(60)91245-2. [DOI] [PubMed] [Google Scholar]
  10. PATTERSON D. S. Qualitative and quantitative changes observed in the free alpha-amino nitrogen fraction of Tenebrio molitor pupal tissues during metamorphosis. Biochem J. 1957 Apr;65(4):729–735. doi: 10.1042/bj0650729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PRESCOTT D. M., BENDER M. A. Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. Exp Cell Res. 1962 Mar;26:260–268. doi: 10.1016/0014-4827(62)90176-3. [DOI] [PubMed] [Google Scholar]
  12. QUASTEL J. H., BICKIS I. J. Metabolism of normal tissues and neoplasms in vitro. Nature. 1959 Jan 31;183(4657):281–286. doi: 10.1038/183281a0. [DOI] [PubMed] [Google Scholar]
  13. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUMEL Action of actinomycin D on animal cells and viruses. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1238–1245. doi: 10.1073/pnas.48.7.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SUNG S. C., QUASTEL J. H. SARCOMYCIN INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN EHRLICH ASCITES CARCINOMA CELLS. Cancer Res. 1963 Oct;23:1549–1554. [PubMed] [Google Scholar]
  15. SWANN M. M. The control of cell division; a review. I. General mechanisms. Cancer Res. 1957 Sep;17(8):727–757. [PubMed] [Google Scholar]
  16. Steinberg R. A., Thom C. Mutations and Reversions in Reproductivity of Aspergilli with Nitrite, Colchicine and d-Lysine. Proc Natl Acad Sci U S A. 1940 Jun 15;26(6):363–366. doi: 10.1073/pnas.26.6.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TAYLOR J. H. Nucleic acid synthesis in relation to the cell division cycle. Ann N Y Acad Sci. 1960 Oct 7;90:409–421. doi: 10.1111/j.1749-6632.1960.tb23259.x. [DOI] [PubMed] [Google Scholar]
  18. TENENHOUSE A., QUASTEL J. H. Amino acid accumulation in Ehrlich ascites carcinoma cells. Can J Biochem Physiol. 1960 Nov;38:1311–1326. [PubMed] [Google Scholar]
  19. THOMSON R. Y., SMELLIE R. M., DAVIDSON J. N. The nucleic acid metabolism of animal cells in vitro. II. Characteristics of the in vitro pattern. Biochim Biophys Acta. 1958 Aug;29(2):308–320. doi: 10.1016/0006-3002(58)90190-2. [DOI] [PubMed] [Google Scholar]
  20. WANG D. Y., GREENBAUM A. L., HARKNESS R. D. The effect of colchicine on the acid-soluble ribonucleotides of normal and regenerating rat liver. Biochem J. 1963 Jan;86:62–64. doi: 10.1042/bj0860062. [DOI] [PMC free article] [PubMed] [Google Scholar]

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