2D/3D	Two-/Three-Dimensional Cell Culture
3Rs	Replacement, Reduction, and Refinement
ADME	Absorption, Distribution, Metabolism, and Excretion
AI	Artificial Intelligence
CFU	Colony-Forming Unit
DA	Defined Approach
DDI	Drug–drug interaction
DILI	Drug-Induced Liver Injury
EMA	European Medicines Agency
FDA	Food and Drug Administration
HCI	High-Content Imaging
hESC	Human Embryonic Stem Cell
hiPSC/iPSC	(Human) Induced Pluripotent Stem Cell
hiPSC-CM	hiPSC-Derived Cardiomyocyte
HSC	Hematopoietic Stem Cell
qHTS	Quantitative High-Throughput Screening
IATA	Integrated Approaches to Testing and Assessment
ISSCR	International Society for Stem Cell Research
LDH	Lactate Dehydrogenase
ML	Machine Learning
MPS	Microphysiological System
MTT	3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
NAMs	New Approach Methodologies
NIH	National Institutes of Health
NRU	Neutral Red Uptake
OECD	Organisation for Economic Co-operation and Development
PBPK	Physiologically Based Pharmacokinetic (Modelling)
PFAS	Polyfluoroalkyl Substances
PI	Propidium Iodide
PSC	Pluripotent Stem Cell
QIVIVE	Quantitative In Vitro–In Vivo Extrapolation
QSAR	Quantitative Structure–Activity Relationship
ROS	Reactive Oxygen Species
RTCA	Real-Time Cell Analysis
SRB	Sulforhodamine B
STS	Sequential Testing Strategy
tcpl	ToxCast Pipeline for Curve Fitting
Tox21/ToxCast	U.S. Toxicology Data Programs for High-Throughput Screening