FIG. 6.

Correlation between orange autofluorescence (575 to 605 nm) and SSC for standard beads, P. infestans sporangia and zoospores, other fungal spores, and pollen analyzed in the PAS-III. We excluded 6-μm beads since these were fluorescent standards and could not be shown on the same scale. Agrostis pollen also could not be shown on the same scale (orange fluorescence [FL] MCN > 3,500). MCN, median channel number (considered to be more reliable than the peak channel number for asymmetric or irregular distributions which were often found for fluorescence intensity). The linear least-squares best-fit line for beads was calculated in MS Excel (see Fig. 2).