TABLE 2.

Effectiveness of Calcofluor white M2R for differentiation of P. infestans sporangia from other fungal spores and pollen grains^a

Source	% Particles falling within gatesb defined for P. infestans sporangia when stained with Calcofluor white M2R at:
	0 μM	10μM	100 μM	1 mM
Alternaria sp.	0	0	0	0
Blumeria sp.	5, 47, 65	0, 0, 0	0, 1, 2	0
Brown rust	0, 0	0	0, 0	NT
Crown rust	0, 2	0	0, 0	NT
Poa annua pollen	0, 0, 1	0, 0, 3	0, 0, 1	0, 4, 5
P. infestans sporangia	62, 67, 78, 87, 90, 92, 92, 93	69, 71, 77, 89, 89, 91, 92	77, 79, 81, 84, 85, 85, 91	36, 55, 67, 72

^aData were analyzed by using multiple gating based on four parameters measured in the PAS-III: FSC, UV, and green and orange fluorescence. Figures separated by commas indicate experiments conducted on different days. NT, not tested.

^bThe logical gating used was R1 and R2, where R1 and R2 were polygonal regions drawn from two-dimensional density plots of FSC versus green fluorescence and UV versus orange fluorescence, respectively, derived from data for sporangial suspensions. In the case of the other spores and pollens, data were first gated by using polygonal regions drawn from FSC versus green fluorescence plots for the individual data sets, in order to remove background particles.