Biodegradation of SDS by Pseudomonas sp. strain AE-A. Growth (mean and standard deviation, n = 4 [○]) and residual surfactant concentration (mean and standard deviation, n = 3 [▴]) were measured with BS medium supplemented with 1 g of surfactant/liter in batch cultures. Samples were removed at the times indicated by the arrows and analyzed by gel zymography for specific alkylsulfatases. Designations above arrows, e.g., AP3, indicate alkylsulfatase bands present in the sample. O.D.540, optical density at 540 nm; concn., concentration.