FIG. 4.

Examples of PAGE zymograms of primary alkylsulfatases in Pseudomonas sp. strains AE-A and AE-D. Migration of proteins is toward the bottom (anode). Gel 1, strain AE-A grown in nutrient broth and gel stained with 2-butyloctyl sulfate; gel 2, strain AE-A grown on SDS and gel stained with SDS; gels 3 and 4, strains AE-A and AE-D, respectively, both grown on and gels stained with 2-butyloctyl sulfate. Full details of the conditions under which each band was produced are shown in Table 1. “Bands” visible at the top and bottom of each gel are artifacts caused by refraction of light from the ends of the rod gels.