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Production of DP1 alkylsulfatase (mean and standard deviation, n = 2 [▪]) during growth (mean and standard deviation, n = 4 [○]) of Pseudomonas sp. strain AE-D on 2-butyloctyl sulfate (initially 1 g/liter) in batch cultures. Enzyme activity was measured in duplicate as described in the text and is expressed as units per milliliter of extracts of cells which were harvested and resuspended at 50 times the culture cell density prior to disruption. O.D.540, optical density at 540 nm.
