TABLE 1.
Alkylsulfatase specificities based on gel zymograms of cell extracts of alkyl sulfate-degrading bacteria grown with different substrates
| Pseudomonas strain | Enzyme band designationa | Occurrence of bands on gel zymograms with the following growth substrate and gel substrateb:
|
|||||
|---|---|---|---|---|---|---|---|
| 2BOS
|
SDS
|
Nutrient broth
|
|||||
| SDS | 2BOS | SDS | 2BOS | SDS | 2BOS | ||
| AE-A | AP1 (0.27) | + | + | + | + | + | + |
| AP2 (0.24) | − | + | − | − | − | − | |
| AP3 (0.55) | − | − | + | − | − | − | |
| AE-D | DP1 (0.27) | − | + | NG | NG | − | − |
| C12B | P1 (0.25) | NG | NG | + | − | + | − |
| P2 (0.38) | NG | NG | + | − | − | − | |
a
Numbers in parentheses are electrophoretic mobilities relative to that of the bromophenol blue tracker dye.
b
+, band was present on gel; −, band was absent from gel; NG, no growth. 2BOS, 2-butyloctyl sulfate. Growth substrates are listed above gel substrates.